importing and trimming reads
Mapping to Sigenae 8
CSV export (raw)
Going with 1x V coverage and 60% Horizontal cov
and length of consensus > 100
= 7979 genes
-
Enrichment analysis
note 82312 sequences in Sig 8
Best HitÂ
JOIN siglist ^ to full Sigenae Best Hit list to IDs for DAVID
uploading background (best hits)
DAVID FILES
taking unmapped reads and mapping to OSHV (NC_005881)
Taking left-overs and de novo
blasting on SP